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- www.nhm.ac.uk
  
  STOCK SOLUTIONS
  
  1. STOCK SOLUTIONS. Prepared by Mrs Andie Hall. 3M NaOAc pH 5.2. 49.22g molecular biology grade ...
  
  2024-07-21
  
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- www.bio.utk.edu
  
  UT Knoxville | Mycology Lab - Biological Sciences | College of Arts & Sciences
  
  The University of Tennessee, Knoxville was founded in 1794 and was designated the state land-grant institution in 1879. The University now has nearly 26,000 students and 400 ...
  
  2024-07-21
  
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Plant Protein Isolation Rapid isolation of protein for SDS-PAGE analsyis GUS Assays

日期：2024-07-20

Plant Protein Isolation Rapid isolation of protein for SDS-PAGE analsyis (Essentially the same protocol as that described for GUS Assays) A. Method for ~1g or more of tissue. 1. Label all tubes. Prepare solutions and have ready at hand. 2. Remove the tiss...

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DNA Extraction Protocol 2 - University of Hawaii System

日期：2024-07-21

DNA Extraction Protocol #2 [back] This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria 1. Pellet cells from 10 ml overnight cultures in BHI or LB and wash in 5 ml of 0.1X SSC....

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DNA Isolation Protocol - MIT - Massachusetts Institute of Technology

日期：2024-07-25

1. Each tail should be in a clean eppendorf tube. 2. Add 500µl of tail lysis buffer containing Proteinase K (PK) to each tube. 3. Incubate tail samples in 50-60C water bath overnight. 4. Add 250µl saturated (6M) NaCl to each tube. 5....

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Genomic DNA extraction - BouillaBase.org Home Page

日期：2024-07-21

Genomic DNA Extraction from fish fin clips Previously we have used proteinase K digestion followed by phenol: chloroform extraction (see below). More recently we have had good luck with Qiagen's DNeasy protocol: DNeasy protocol. The primary pages from the...

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Chromosomal DNA isolation from E. coli - OpenWetWare

日期：2024-07-19

Curators Torsten Waldminghaus Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently discussing the idea of protocol curators. Plea...

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Isolation of DNA from Wheat - Department of Chemistry | Indian Institute of Technology Khara

日期：2024-07-24

Experiment 3 Isolation of DNA from Wheat Reagents: • DNA extraction buffer: 25mM Tris-HCl(pH = 8.0), 50 mM EDTA, 0.5% SDS (Before using, add β-mercaptocthanol to a final concentration of 2%) • Phenol-chloroform (1:1) • 3 M sodium acetate (pH 5.2)...

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I. General methods - The University of Oklahoma's Advanced Center for Genome Techno

日期：2024-07-21

I. General methods A. Phenol extraction of DNA samples Phenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated phenol is added to an aqueous DNA sample in a microcentrifuge tube. The mixture is ....

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