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- obprotocol.blogspot.com
  
  Online Biological Protocol: 3M sodium acetate preparation
  
  17 Oct 2008 ... 3M sodium acetate is used to precipitate DNA or RNA from ethanol. For 50 ml, 20.412 g Sodium ...
  
  2024-07-26
  
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- www.protocol-online.org
  
  sodium acetate - Biochemistry - Protocol Online - Your lab's reference book
  
  hello I know that just as NaCl it (0.3M, pH 5) precipitates nucleic acid w/o cristallizing, but it inhibits the Klenow fragment... NH4Ac (2-2.5M) is unable to precipitate ... The purpose of adding salts is to neutralize the charge on the sugar-phosphate b
  
  2024-07-26
  
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Online Biological Protocol

日期：2024-07-20

Preparation of 3M sodium acetate (50ml):3M sodium acetate is used to precipitate DNA or RNA from ethanol. For 50 ml, 20.412 g Sodium acetate [ CH3COONa•3H2O, trihydrate] ~6 ml acetic acid to pH, adjust the pH to 5.2 using Glacial acetic acid....

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Ethanol Precipitation to clean DNA

日期：2024-07-22

How to "Clean" DNA (ethanol precipitation protocol) Anytime DNA has to be "cleaned", this means that all of the proteins, small nucleic acids and salts need to be removed from a solution that contains sizeable dsDNA. To do this, we have to precipitate the...

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Buffers and Standard Solutions - Ivan J Delgado Orlic

日期：2024-07-23

BUFFERS AND STANDARD SOLUTIONS Last Update: December 2006 from Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory) BUFFER AND SOLUTION ......

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QIAEX II Handbook - QIAEX II Agarose Gel Extraction Protocol

日期：2024-07-24

12 QIAEX II Handbook 02/99 Protocol QIAEX II Agarose Gel Extraction Protocol This protocol is designed for the extraction of 40-bp to 50-kb DNA fragments from 0.3–2% standard or low-melt agarose gels in TAE or TBE buffers. Notes: • The yellow color of Buf...

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