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日期:2024-08-11
Santa Cruz Biotechnology, Inc. Antibodies - World Leader in Antibody and siRNA
Products. ... Optimal antibody concentration should be determined by titration. ...
antibody (Conventional Secondary Antibodies for Western Blotting), diluted to ......
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日期:2024-08-13
Western blotting (also called immunoblotting because an antibody is used to ....
the wash buffer will vary, though typical concentrations are from 0.05 to 0.5% for ......
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日期:2024-08-10
Figure 1 – Effect of Varying Primary Antibody Concentration: Western blot
optimization using R&D Systems rabbit anti-human/mouse/rat RSK1 affinity
purified ......
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日期:2024-08-15
29 Aug 2012 ... Western Blot Protocol ... 1.5 Anti-IgG Secondary antibody. Use one of the
following secondary antibodies according to the species of primary ......
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日期:2024-08-15
19 Oct 2013 ... I purchase primary PPARγ antibody (H-100) is a rabbit polyclonal IgG ... If this
gives a nice blot, go down with the concentration gradually to see ......
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日期:2024-08-14
Solve your western blot problems with these troubleshooting tips, covering
common ... Titrate the antibody to the optimal concentration, incubate for longer
but in ......
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日期:2024-08-13
Western blotting uses specific antibodies to identify proteins that have been
separated ... Please consult our separate protocol for sub-cellular fractionation....
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日期:2024-08-15
General Western blot protocol. ... Procedure for western blotting .... Incubate
membrane with appropriate dilutions of primary antibody in 5% or 2% blocking ......