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- tools.lifetechnologies.com
  
  Optimize antigen and antibody concentrations for Western blots
  
  Because every Western blot involves a combination of antibody and antigen ... Appropriate primary and secondary antibody concentrations depend on each ...
  
  2024-08-10
  
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- www.google.com.tw
  
  Western Blotting Protocol | CST Cell Signaling Technology
  
  For western blots, incubate membrane with diluted primary antibody in either 5% w/v BSA or nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle ...
  
  2024-08-16
  
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Western (Immuno-) Blotting Santa Cruz Biotechnology, Inc

日期：2024-08-11

Santa Cruz Biotechnology, Inc. Antibodies - World Leader in Antibody and siRNA Products. ... Optimal antibody concentration should be determined by titration. ... antibody (Conventional Secondary Antibodies for Western Blotting), diluted to ......

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Overview of Western Blotting | Life Technologies

日期：2024-08-13

Western blotting (also called immunoblotting because an antibody is used to .... the wash buffer will vary, though typical concentrations are from 0.05 to 0.5% for ......

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Western Blot Figures - R&D Systems

日期：2024-08-10

Figure 1 – Effect of Varying Primary Antibody Concentration: Western blot optimization using R&D Systems rabbit anti-human/mouse/rat RSK1 affinity purified ......

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Western Blot Protocol - Abnova

日期：2024-08-15

29 Aug 2012 ... Western Blot Protocol ... 1.5 Anti-IgG Secondary antibody. Use one of the following secondary antibodies according to the species of primary ......

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How much concentration of primary and secondary antibody is ...

日期：2024-08-15

19 Oct 2013 ... I purchase primary PPARγ antibody (H-100) is a rabbit polyclonal IgG ... If this gives a nice blot, go down with the concentration gradually to see ......

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Western blot troubleshooting tips | Abcam

日期：2024-08-14

Solve your western blot problems with these troubleshooting tips, covering common ... Titrate the antibody to the optimal concentration, incubate for longer but in ......

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Western Blotting - Abcam

日期：2024-08-13

Western blotting uses specific antibodies to identify proteins that have been separated ... Please consult our separate protocol for sub-cellular fractionation....

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Western blot protocol - Abcam

日期：2024-08-15

General Western blot protocol. ... Procedure for western blotting .... Incubate membrane with appropriate dilutions of primary antibody in 5% or 2% blocking ......

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