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- www.med.nyu.edu
  
  E. coli Calcium Chloride competent cell protocol Then ...
  
  E. coli Calcium Chloride competent cell protocol. 1. Inoculate a single colony into 5mL Lb in 50mL falcon tube. Grow. O/N @ 37°C. 2. Use 1mL to inoculate ...
  
  2025-12-12
  
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- openwetware.org
  
  Preparing chemically competent cells - OpenWetWare
  
  Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating pipettor 5ml pipette Preparation Grow a 5ml overnight culture of cells i
  
  2025-12-09
  
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TOP10 chemically competent cells - OpenWetWare

日期：2025-12-07

Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells. See Bacterial ...

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competent cells Methods, Protocols and Troubleshootings

日期：2025-12-12

Top10 and DH10B need IPTG for induction? - (reply: 1) Checking for efficieny competent cells - (reply: 1) freezing competent cells without dry ice or liquid N - (reply: 3) Expansion of commercial competent bacterial cell lines? - (reply: 2) What is your f...

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Protocol Competent cell preparation - iGEM 2010

日期：2025-12-08

Competent cell preparation. A. Preparing glassware and media eliminate detergent. 1. Autoclaving glassware filled 3/4 with DD-H2O to remove most detergent....

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Making Calcium Competent Cells

日期：2025-12-10

Streak out frozen glycerol stock of bacterial cells (Top10, DH5α, etc.) ... Prepare starter culture of cells ... autoclaved for the next time you make competent cells....

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An improved system for competent cell preparation and high efficiency plasmid transformation using d

日期：2025-12-12

Competent cell preparation and plasmid transformation 116 Determination of their early log phase is important. The cells were pelleted by centrifugation at 8000 rpm for 1 min at 4ºC, then resuspended in one-half volume (20 ml) of sterile cold TB (CaCl2) s...

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