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日期:2024-07-08
www.molbi.de, molbi, Untergasser's Lab, Molecular Biology, Cloning, Protocol, Media ... Preparation of Chemical Competent Cells by A. Untergasser (contact address and download at www.untergasser.de/lab) Version: 1.0 - Print Version (.PDF)...
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日期:2024-07-10
Mix 500 mL of LB media with 7 g of Agar (Autoclave). Cool to ~55-65oC prior to
pouring. The addition of antibiotics ......
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日期:2024-07-04
28 Jan 2014 ... Preparation of chemically competent cells (protocol) · Preparation of
electrocompetent cells (protocol)....
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日期:2024-07-08
Adapted from protocol given by Joe Graber Amber Pollack 7/6/05.
PREPARATION OF COMPETENT CELLS (E. coli)....
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日期:2024-07-05
4 Apr 2013 ... Preparation. Grow a 5ml overnight culture of cells in LB media. In the morning,
dilute this culture back ......
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日期:2024-07-05
Materials. TB buffer. 10 mM HEPES pH 6.7; 15 mM CaCl2; 55 mM MnCl2; 250
mM KCl. Mix all component except ......
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日期:2024-07-09
Competent cell preparation and plasmid transformation. 114. Wuhan, 4300074,
China. Tel: 86 27 87556214. Fax: 86 ......