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日期:2024-08-25
2012年5月15日 - This protocol permits the mutation scanning of PCR products by high-resolution DNA melting analysis requiring the inclusion of a saturating ......
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日期:2024-08-24
Sponsored,vendor-submitted protocol Published in BioTechniques Protocol Guide 2007 (p.67). DNA melting (dissociation) analysis is widely used for the ......
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日期:2024-08-23
High resolution melt (HRM) analysis is an extension of previous DNA .... The assay design and analysis principles outlined in this protocol are valid for other ......
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日期:2024-08-22
melting curve acquisition Melting analysis was performed on the LightCycler or on a high-resolution melting instrument (HR-1; Idaho Technology), as described previously for labeled primers (10). After amplification, the samples were heated ......
Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis : Abstract : N
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日期:2024-08-27
This protocol permits the simultaneous mutation scanning and genotyping of PCR products by high-resolution DNA melting analysis. This is achieved using asymmetric PCR performed in the presence of a saturating fluorescent DNA dye and unlabeled oligonucleot...
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日期:2024-08-26
High Resolution Melt (HRM) analysis is a powerful technique in molecular
biology for the detection of .... the use of SYBR Green I for HRM, claiming that
substantial protocol modifications are required....
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日期:2024-08-26
1 Aug 2011 ... Learn the basics of High Resolution Melt Analysis (HRM), applications, important
... Run amp + high resolution melt protocol on CFX96 or CFX384 98°C for 2 min
98°C for 2-5s 55-60°C ......