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日期:2024-07-20
The melting temperature of the amplicon at which the two DNA strands come apart is entirely predictable. ... HRM is a more cost effective solution and the high resolution element not only allows the determination of homo and heterozygosity, it also resolv...
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日期:2024-07-20
High Resolution Melting (HRM) Analysis; is a powerful technology for SNP genotyping, mutation scanning and sequence scanning in DNA samples. Learn how HRM technology works, how HRM primers are designed, advantages and applications of HRM...
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日期:2024-07-16
Application Guide High Resolution Melt Analysis Contents Page Introduction 3 Overview of the Melting Profile Principle 3 The Intercalating Dye Non-saturating dyes 4 Saturating dyes 4 Release-on-demand dyes 4 Instruments and Software 5 Summary of the ......
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日期:2024-07-20
High Resolution Melting (HRM) analysis is a post-PCR technique used to identify genetic variation in nucleic acid sequences. It is often used to screen large numbers of potential variant sequences quickly and inexpensively as a precursor to ot...
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日期:2024-07-22
ABSTRACT High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied ......
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日期:2024-07-23
HRM analysis can also be used to detect methylation differences between genes; this is known as methylation-specific high resolution melting (MS-HRM) ......
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日期:2024-07-16
In the 1960s, melting of double-stranded DNA was monitored by UV absorbance (hyperchromic effect). Analysis required µg amounts of DNA, and often took ......