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日期:2024-07-21
In the 1960s, melting of double-stranded DNA was monitored by UV absorbance (hyperchromic effect). Analysis required µg amounts of DNA, and often took ......
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日期:2024-07-18
For HRM experiments, data are generally collected at narrower temperature increments than for standard melt curve protocols, ... Temesvári M et al. (2011). High-resolution melting curve analysis to establish CYP2C19∗2 single nucleotide polymorphism PMID: ...
Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validatio
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日期:2024-07-17
1. Hum Mutat. 2009 Jun;30(6):899-909. doi: 10.1002/humu.21004. Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner. van der Stoep N, van Paridon CD...
BMC Genetics | Full text | A Strategy to Setup Codominant Microsatellite Analysis for High-Resolutio
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日期:2024-07-21
High resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA. The measurement is immediately following PCR in a one-step, closed-tu...
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日期:2024-07-23
melting curve acquisition Melting analysis was performed on the LightCycler or on a high-resolution melting instrument (HR-1; Idaho Technology), as described previously for labeled primers (10). After amplification, the samples were heated ......
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日期:2024-07-21
Correspondence and requests for reprints should be addressed to: Michael H. Cho, MD, Channing Laboratory, Brigham and Women’s Hospital, Boston, MA 02115. ... High-resolution melting curve analysis is an accurate method for mutation detection in genomic .....
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日期:2024-07-20
Abstract Background: High-resolution melting curve analysis is an accurate method for mutation detection in genomic DNA. Few studies have compared the performance of high-resolution DNA melting curve analysis (HRM) in genomic and whole ......