search:high resolution melting protocol相關網頁資料

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日期:2024-07-19
High Resolution Melting (HRM) analysis is a relatively new, post-PCR analysis method used to identify variations in nucleic acid sequences. The method is ......
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日期:2024-07-22
High Resolution Melting Analysis (HRM or HRMA) is a recently developed technique ... A Low Resolution Melt curve is produced by increasing the temperature ......
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日期:2024-07-18
The melting temperature of the amplicon at which the two DNA strands come apart is entirely predictable. ... HRM is a more cost effective solution and the high resolution element not only allows the determination of homo and heterozygosity, it also resolv...
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日期:2024-07-18
This protocol permits the mutation scanning of PCR products by high-resolution DNA melting analysis requiring the inclusion of a saturating intercalating dye in the PCR mix without labelled probe. During a scanning process, fluorescent melting curves of P...
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日期:2024-07-16
A High-Resolution Melting Protocol for Rapid and Accurate Differential Diagnosis of Thyroid Nodules Irene Mancini ⁎, Pamela Pinzani ⁎, Cinzia Pupilli †, Luisa Petrone †, Maria Laura De Feo †, Lapo Bencini ‡, Mario Pazzagli ⁎, Gianni Forti †, Claudio Orlan...
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日期:2024-07-21
The base composition of PCR products derived from sodium bisulfite-modified templates is methylation dependent. Hence, methylated and unmethylated, PCR products show different melting profiles when subjected to thermal denaturation. The methylation-sensit...