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Rational design of a high-affinity, fast, red calcium indicator R-CaMP2 : Nature Methods : Nature Pu
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日期:2024-07-03
(a) Experimental setup for recording air-puff whisker stimuli–evoked calcium transients. (b) Sensory-evoked calcium transients in vivo. Left, fluorescence changes were imaged from eight regions of interest (# 1– # 8). Right, calcium transient traces. Vert...
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日期:2024-07-06
Chlorophyll fluorescence is light re-emitted by chlorophyll molecules during return from excited to non-excited states and used as indicator of photosynthetic energy conversion in higher plants, algae and bacteria. Excited chlorophyll dissipates the absor...
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日期:2024-07-03
Test House Directory - for test houses, test equipment manufacturers and distributors, published by Concorde Publishing on behalf of the Society of Environmental Engineers ... Welcome to TestHouseDirectory.com This is the online companion to the highly .....
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日期:2024-07-08
In the other model experiment we studied the temperature-induced heat denaturation of the MD-W501+ construct by monitoring tryptophan fluorescence. We directly followed the stability of the protein structure around the W501 side chain located in the relay...
Ultrasensitive fluorescent proteins for imaging neuronal activity : Nature : Nature Publishing Group
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日期:2024-07-07
Abstract • Introduction • GCaMP protein engineering • Imaging neuronal populations in mouse V1 • Calcium transients in dendritic spines• ... This video shows simultaneous recording of spikes and GCaMP6s fluorescence signal of a L2/3 neuron in the visual c...
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日期:2024-07-03
Figure 19.3.5 Fast confocal recording of spontaneous Ca 2+ sparks in a rat ventricular myocyte. Single myocytes were isolated from rat hearts and loaded with fluo-4 AM (F14201, F14202, F14217, F23917). Images of loaded cells were obtained with a PerkinElm...
Imaging Activity in Neurons and Glia with a Polr2a-Based and Cre-Dependent GCaMP5G-IRES-tdTomato Rep
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日期:2024-07-05
Figure 2. Recording Spontaneous Calcium Activity in Intact, Awake, and Behaving Neonates (A) tdTomato expression in Emx1-IRES-Cre; PC::G5-tdT. (B–G) Frames from a movie demonstrating spontaneous transients throughout the cortex. Scale bar, 1 mm. (B ......
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日期:2024-07-08
Imaging of mouse motor cortex (M1) expressing the genetically-encoded calcium indicator GCaMP3 through a cortical window. After 72 days of GCaMP3 expression, large fluorescence transients can be seen in many neurons that are highly correlated with mouse ....