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  1 Nomenclature & Abbreviations; 2 Methylation Issues in E. coli; 3 Commonly used strains ..... DM1 (Invitrogen) ... From a GIBCO BRL list of competent cells.
  
  2024-08-01
  
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  Nomenclature & Abbreviations A listed gene name means that gene carries a loss of function mutation, a Δ preceding a gene name means the gene is deleted. If a gene is not listed, it is not known to be mutated. Prophages present in wt K-12 strains (F, λ, e
  
  2024-08-02
  
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Pro 5-alpha, JM109 and HB101 Competent Cells - Promega

日期：2024-07-30

In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two ......

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Bacterial Strain JM109 - Promega

日期：2024-08-01

JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/ white screening. ... Pro 5-alpha, JM109 and HB101 Competent Cells ......

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Manual: JM109 Competent Cells

日期：2024-08-02

JM109 competent cells (colorless tubes). 5 × 0.2 ml. ≥1 × 108 ... following the protocol outlined below. Following ......

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JM109 Competent Cells for routine DNA plasmid production | Sigma ...

日期：2024-08-05

Sigma-Aldrich offers Sigma-J3895, JM109 Competent Cells for your research needs. ... Protocols & Articles. 3....

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SLiCE: a novel bacterial cell extract-based DNA cloning method

日期：2024-07-30

Bacteria strains The following laboratory E. coli strains were used: DH10B (Invitrogen), JM109 (Promega), BL21(DE3) (Invitrogen), BLR(DE3) (Novagen) and ER2566(NEB). The DH10B derived E. coli strain PPY was constructed by Suicide Plasmid Based Genome ......

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pGAPZ A, B, and C pGAPZ A, B, and C - Gene Synthesis service-0,20€/bp-Peptide Synthesis

日期：2024-07-31

vii Important Information Contents This manual is included with the following catalog numbers: Catalog no. Item Contents V200-20 pGAPZ A, B, and C 20 µg each vector, lyophilized X-33/pGAPZ B/lacZ Pichia strain expressing β-galactosidase V205-20 pGAPZα ......

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transformation Methods, Protocols and Troubleshootings

日期：2024-08-03

Transformation of non-competent E. coli? - Any shortcut for preapring competent cells? (reply: 2) small transformant colonies - (reply: 1) Chemical transformation in 96 well MTP - How to make competent cells in MTPs (reply: 2) Competent Cells - (reply: 1)...

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Simple Cloning: direct transformation of PCR product (DNA multimer) to Escherichia coli and Bacillus

日期：2024-07-29

8 177 Figure legends 178 Fig. 1. The scheme of Simple Cloning. First, two 3 and 5 overlapped insertion and 179 vector fragments are generated by regular PCR. Second, DNA multimer is formed by 180 POE-PCR without primers and with a prolonged ......

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