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- pcrlab.blogspot.com
  
  生物技術學: RT-PCR、PCR、Real time-PCR
  
  2009年5月14日 ... 先簡單介紹聚合鏈反應(PCR, polymerase chain reaction)吧！ ... 至於即時RT-PCR (real time RT-PCR)則是利用非常敏感的螢光染劑(例如Sybr ... 釋出的螢光指數，由於聚合連鎖反應會造成DNA成對數性的增加(每個循環就增加2倍
  
  2024-07-25
  
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- www.genedragon.com.tw
  
  PCR 原理,應用和Troubleshooting
  
  逆轉錄PCR(RT-PCR)：將目標細胞內的RNA逆轉錄為DNA，再進行聚合酶反應，這是一種分析細胞內RNA表現的方法之一。即時PCR(real-time PCR)：PCR過程中 ...
  
  2024-07-24
  
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Annealing temperature for PCR - PCR, RT-PCR and Real-Time PCR

日期：2024-07-27

What are your melting temperatures? You can use the following equation to determine annealing temperature: Ta = average melting temperature of both forward and reverse primers then subtract 3 degrees from the total. However, its best to run a gradient PCR...

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Tm - Melting Temperature of PCR products | Real Time qPCR Tutorial & Instruments review

日期：2024-07-27

Tm is the melting temperature of the PCR product. Since the DNA helix melts in a temperature range rather that at one very specific temperature, Tm is defined as the temperature at which 50% of the helices are dissocited. Tm is useful for monitoring your ...

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Real-Time PCR Detection Systems | Life Science Research | Bio-Rad

日期：2024-07-28

Life Science Research Products PCR Amplification Antibodies Flow Cytometry Transfection Electrophoresis and Blotting Imaging Systems & Software ... Overview Bio-Rad real-time PCR systems combine a thermal cycler and optical reaction module for singleplex ...

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Real-Time PCR: Current Technology and Applications | Book

日期：2024-07-23

A comprehensive guide to the most up-to-date real-time PCR technology and applications. The latest PCR platforms, fluorescent chemistries, validation software, data analysis, internal and external controls,clinical diagnostics, biodefense, RNA expression ...

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Real Time PCR Primer Sets !!!

日期：2024-07-27

Database of real time PCR primer sets for quantitative PCR ... Quantitative real time PCR is an important step for the validation of expression data generated by microarray analysis and other genomics techniques....

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CFX Connect™ Real-Time PCR Detection System | Life Science Research | Bio-Rad

日期：2024-07-26

Efficient Optimization Determining the optimal temperature for primer annealing is crucial for efficient and specific amplification of product. With the CFX Connect system's thermal gradient feature, you can determine the optimal temperature for primer an...

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