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日期:2024-08-11
Reverse Transcription Priming The choice of primers to be used to initiate reverse transcription can greatly affect RT-qPCR results. For one-step RT-qPCR, gene-specific primers are used. When performing a two-step assay, a reverse gene-specific primer, ol...
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日期:2024-08-08
15 Apr 2015 ... Real-Time qRT-PCR. Introduction. Real-Time qRT-PCR. (Real-Time Quantitative
Reverse Transcription PCR) is a major development of PCR ......
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日期:2024-08-11
J Mol Endocrinol. 2005 Jun;34(3):597-601. Quantitative real-time RT-PCR--a
perspective. Bustin SA(1), Benes V, Nolan T, Pfaffl MW. Author information:...
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日期:2024-08-10
qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for
the detection and quantification of RNA targets and is being utilized increasingly
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日期:2024-08-06
1 Jun 2005 ... The real-time reverse transcription polymerase chain reaction (RT-PCR) uses
fluorescent reporter molecules to monitor the production of ......