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日期:2025-01-17
Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. RT-PCR is often confused with real-time polymerase chain .....
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日期:2025-01-23
RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA ......
Leukemia - Evaluation of candidate control genes for diagnosis and residual disease detection in leu
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日期:2025-01-18
Figure 1. Schematic diagram of the three selected CG transcripts covered by the EAC RQ-PCR sets. ABL EAC set: ENF1003, ENPr1043, ENR1063; B2M EAC set: ENF1302, ENPr1342, ENR1362 set, and GUS EAC set: ENF1102, ENPr1142, ENR1162. The ......
Retrovirology | Full text | Cross-clade simultaneous HIV drug resistance genotyping for reverse tran
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日期:2025-01-21
Figure 1. Preparation and sequencing of samples. (A) Plasma is isolated from whole blood from up to 96 samples. (B) Viral RNA is isolated from up to 1 ml of plasma. (C) Viral RNA is used in a one-step RT-PCR amplification of a 2.8 kb region of the pol gen...
Leukemia - Standardization and quality control studies of /`real-time/' quantitative reverse transcr
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日期:2025-01-18
Leading Article Leukemia (2003) 17, 2318–2357. doi:10.1038/sj.leu.2403135 Published online 9 October 2003 Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts fo...
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日期:2025-01-18
The Applied Biosystems® StepOne and StepOnePlus Real-Time PCR Systems offer intuitive software, Fast (...
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日期:2025-01-17
Home > Support > qRT-PCR & PCR Array FAQs : qRT-PCR & PCR Array Sample Preparation How to maximize the quality and yields of your RNA preps Reverse Transcription Choosing the right primers and controls Quantitative PCR Designing and executing an ......
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日期:2025-01-17
nested polymerase chain reaction use of the PCR in series such that a specified piece of DNA is amplified and then a portion contained within the first piece is amplified further; used where extremely low amounts of DNA are present, or where there are pro...