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Diagnosis of Respiratory Syncytial Virus Infection: Comparison of Reverse Transcription-PCR to Viral
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日期:2024-09-05
Diagnosis of respiratory syncytial virus (RSV) during acute infection in adults is difficult because of the poor sensitivity of viral culture and antigen detection. A recently developed single-tube nested reverse transcription-PCR (RT-PCR) was compared to...
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日期:2024-09-10
BIOMEDICAL RESEARCH TECHNIQUES (MEDSCI 720) PCR Real-time qPCR In situ hybridisation Srdjan Vlajkovic Department of Physiology ... Transcript 1. BIOMEDICAL RESEARCH TECHNIQUES (MEDSCI 720) PCR Real-time qPCR In situ ......
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日期:2024-09-05
Back to top Commonly used terms in PCR Basic terms used in data analysis are given below. For more information on data analysis, refer to the recommendations from the manufacturer of your real-time cycler. Data are displayed as sigmoidal-shaped amplificat...
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日期:2024-09-10
Basic Principles When performing qPCR, a fluorescent reporter dye is used as an indirect measure of the amount of nucleic acid present during each amplification cycle. The increase in fluorescent signal is directly proportional to the quantity of exponent...
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日期:2024-09-11
In the PCR procedure trace amounts of DNA can be quickly and repeatedly ... Simple and effective: the PCR principle ... method known as reverse transcription PCR (RT-PCR). For the....
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日期:2024-09-10
行動版 - Our results justify the future use of this RT-PCR methodology to examine new estrogen-regulated genes ......
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日期:2024-09-09
The procedure of RT-PCR follows the regular PCR procedure, but the DNA is quantified after each round of .... RT-PCR principle is based on the properties of the PCR reaction kinetics....