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日期:2024-08-09
Protocols Agarose-Formaldehyde Electrophoresis (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School) RNA electrophoresis under denaturing conditions in 2.2M formaldehyde is performed according to Maniatis et al., (1982 .....
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日期:2024-08-03
A day without electrophoresis is very rare in molecular biology labs, because this technique is the standard method used for analyzing, identifying and purifying fragments of DNA. It is also used for separating and analyzing RNAs and oligonucleotides....
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日期:2024-08-02
Current methods of analytical RNA electrophoresis are based on the utilization of either complicated laboratory instrumentation or toxic, carcinogenic, or expen ... Fig. 1. RNA electrophoresis in 1× TAE buffer. (A) Influence of the in-sample formamide con...
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日期:2024-08-06
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about RNA yield. ... Generally, at least 200 ng of RNA must be loaded onto a denaturing agarose gel in order to ...
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日期:2024-08-05
INTRODUCTION Perhaps the most important and certainly the most often used technique in RNA analysis is gel electrophoresis. This technique is generally applicable for RNA detection, quantification, purification by size, and quality assessment....
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日期:2024-08-08
V.B.2 ELECTROPHORESIS OF RNA THROUGH GELS CONTAINING FORMALDEHYDE 1. Prepare gel-running buffer and formaldehyde. a. 5 X Gel-running buffer: 0.2 M morpholinopropanesulfonic acid (MOPS) (pH 7.0) 50 mM sodium acetate 5 mM EDTA (pH 8.0)...
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日期:2024-08-02
Comparison page for DNA and RNA marker for electrophoresis. ... DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples....
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日期:2024-08-02
Products for DNA and RNA electrophoresis ... Automated DNA/RNA Purification Manual DNA Extraction Kits PCR products DNA/RNA Electrophoresis FastGene Gel Pic Imaging System FastGene FAS Digi...