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日期:2024-09-04
IV.G.5 QUANTITATIVE RT-PCR Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of mRNA expressed from endogenous genes, and transfected genes of either stable or transient...
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日期:2024-09-02
Dye Excitation Emission Application Acridine 362 462 Primer Labeling AMCA-S 353 437 Primer Labeling BODIPY® 493/503 500 506 Primer Labeling BODIPY® 530/550 534 554 Primer Labeling BODIPY® 558/569 559 568 Primer Labeling BODIPY® 564/570...
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日期:2024-09-04
This protocol describes the detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Xiaowei Wang and Brian Seed (2003) A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Research 31(24): e154; pp.1-8...
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日期:2024-09-01
The ThermoScript RT-PCR System is designed for the sensitive and reproducible detection and analysis of RNA molecules in a two-step process. ThermoScript RT, an avian reverse transcriptase with reduced RNase H activity, is engineered to have higher ......
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日期:2024-09-02
Detection of measles RNA in a clinical sample can provide laboratory confirmation of infection. Real–time RT–PCR (rRT–PCR) and conventional, endpoint RT–PCR to detect measles RNA are performed at CDC. These protocols are available upon request (email prot...
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日期:2024-09-01
內容, Polymerase Chain Reaction(PCR) ◎ What is PCR? 使用耐高溫的DNA Polymerase (Taq polymerase),快速增幅微量DNA的技術。PCR在法醫學上被廣泛 ......
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日期:2024-08-29
在RT-PCR中,一条RNA链被逆转录成为互补DNA,再以此为模板通过PCR ... ②模板DNA与引物的退火(复性):模板DNA经加热变性成单链后,温度降至55℃左右, ......
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日期:2024-08-31
RT-PCR是將RNA的反轉錄(RT)和cDNA的聚合酶鏈式擴增(PCR)相結合的技術。 ... 引物退火溫度的調節,一般在引物設計軟件推薦溫度的上下2 ℃變化尋找最佳 ......