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  TCA Precipitation Methods, Protocols and Troubleshootings
  
  Phosphatase-Protease inhibitors in TCA total protein extraction - (reply: 2) TCA precipitation - (reply: 3) seeking TCA precipitation advice - (reply: 2) Use cold TCA for protein precipitation? - (reply: 3) Problem with TCA precipitation. - can't see pell
  
  2024-07-13
  
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- www.personal.psu.edu
  
  TCA precipitation - www.personal.psu.edu
  
  TCA precipitating small amounts of protein (5/19/00) The following method is based on observations I made while developing a way to do in vitro protein-DNA crosslinking. Some of these crosslinking experiments involved minute amounts of protein, and a TCA
  
  2024-07-13
  
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Other Protocols - TCA protein precipitation

日期：2024-07-13

To Concentrate Proteins for analysis by SDS PAGE 1.Add one tenth of the sample volume of 77% TCA (trichloroacetic acid) to your protein sample. (e.g. to 900ml sample add 100ml TCA or to 1000ml add 111,1ml TCA) 2.Incubate 30 min on ice. 3.Spin in microfuge...

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2. TCA/Acetone Protein Precipitation Protocol

日期：2024-07-16

Proteomics and Mass Spectrometry Facility, BRC, Cornell University Version 3.0 2. TCA/Acetone Protein Precipitation Protocol The following procedure is used to precipitate proteins from freshly prepared cell lysates containing some contaminants, such as s...

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TCA precipitation of proteins - Protein and Proteomics

日期：2024-07-09

I usually use acetone + 0,1 HCl (for better precipitation) and maximal cooling, and the only way to dissolve precipitated protein with good result is to use urea (for example). When using TCA You must know, that in some cases easy to obtain hydrolised pro...

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Solubility of peptides in trichloroacetic acid (TCA) solutions. Hypothesis on the precipitation mech

日期：2024-07-15

1. Int J Pept Protein Res. 1989 Sep;34(3):166-76. Solubility of peptides in trichloroacetic acid (TCA) solutions. Hypothesis on the precipitation mechanism. Yvon M(1), Chabanet C, Pélissier JP. Author information: (1)Dairy Research Section, INRA ......

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Small scale His-Tag purification under nature conditions

日期：2024-07-16

Protein Precipitation Protocols Protein Precipitation Methods to concentrate or eliminate interferences before electrophoresis or protein determination ... TCA-DOC For precipitation of very low protein concentration 1) To one volume of protein solution, a...

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Precipitation techniques Acetone Precipitation Protocol

日期：2024-07-13

Precipitation techniques Purpose: to concentrate the sample and remove small interfering species, such as salts & detergents, for downstream applications e.g. 2D gels. For small amounts of protein (< low microgram) it may be necessary to add a carrier pro...

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Solubilization of trichloroacetic acid (TCA) precipitated microbial proteins via naOH for two-dimens

日期：2024-07-12

1. J Proteome Res. 2003 Jan-Feb;2(1):89-93. Solubilization of trichloroacetic acid (TCA) precipitated microbial proteins via naOH for two-dimensional electrophoresis. Nandakumar MP, Shen J, Raman B, Marten MR. Department of Chemical & Biochemical ......

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Protocol: Precipitation with Trichloroacetic Acid in Acetone: Nevada Proteomics Center: University o

日期：2024-07-10

Protocol: Precipitation with Trichloroacetic Acid in Acetone A method often used for precipitating soluble proteins from plant tissues. Removes salts as well as hydrophilic and lipid soluble contaminants. However, precipitated proteins can be very difficu...

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