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日期:2024-07-13
Tm Calculator. Directions: To calculate the optimal melting temperatures of your
primers, enter each primer's concentration, its 5'-3' sequence, and the salt ......
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日期:2024-07-09
This application calculates the Tm for a primer, and gives instructions on how to
dilute the primer to a desired concentration....
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日期:2024-07-15
Basic Tm Degenerated nucleotides are allowed. Base-Stacking Tm Degenerated
nucleotides are NOT allowed. Primer concentration: nM. Salt concentration:...
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日期:2024-07-10
Basic Melting Temperature (Tm) Calculations. Two standard approximation
calculations are used. For sequences less than 14 nucleotides the formula is:....
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日期:2024-07-09
15 Jul 2010 ... Oligo Calc: Oligonucleotide Properties Calculator ... None of the following melting
temperature (Tm) calculations provide an adjustment for ......
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日期:2024-07-14
Enzyme Finder, DNA Sequences and Maps Tool, Double Digest Finder, PCR
Selection Tool, Tm Calculator, REBASE, NEBcutter, Polbase, NEBuilder, InBase,
......
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日期:2024-07-09
There are a number of different ways to calculate the melting temperature (Tm) of
an oligo. All of these methods will give different results. Please note that these ......