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日期:2024-09-07
Hummon, A. B., Lim S. R., Difilippantonio, M. J., Ried, T. (2007) Isolation and solubilization of protein ......
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日期:2024-09-03
... other proprietary components which facilitate the isolation of a variety of RNA ... TRIzol® Reagent maintains the integrity of the RNA, while disrupting cells ... If you wish to use other protocols for RNA purification, refer to the TRIzol® .... RNA M...
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日期:2024-09-03
I think that discarding all the ethanol off your RNA pellet is one oh the most challenging steps of Trizol RNA extraction. After spinning the pellet down in ethanol, I usually prefer to turn the tube once upside down (very delicately) on a blotting paper,...
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日期:2024-09-06
Agarose gel for determining RNA quality (image attached) - (reply: 1) Visualization of DNA:RNA hybrid on TBE-UREA GEL - (reply: 4) Can´t get rid of DNA contamination in RNA - (reply: 2) RNA gel problem (melted bands) - (reply: 2) RNA extraction and DNase ...
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日期:2024-09-02
Problem Cause Solution Low RNA yield Incomplete lysis and homogenization Use the appropriate method for lysate preparation based on your starting material as described above. Decrease the amount of starting material used. ......
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日期:2024-09-03
TRIZOL® Reagent Cat. No. 15596-018 Size: 200 ml Store at room temperature WARNING: Toxic in contact with skin and if swallowed. Causes burns. After contact with skin, wash immediately with plenty of detergent and water. If you feel unwell, seek medical .....
Promega Notes 94: Cleanup of TRIzol® Reagent-Purified Total RNA Using the PureYield™ RNA Midiprep Sy
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日期:2024-09-05
INTRODUCTION Many researchers use a dual approach for purifying high-quality RNA, which includes: 1) an acid-phe-nol-guanidine thiocyanate reagent, such as TRIzol® reagent, to disrupt the sample and inactivate RNases; and 2) a column purification method t...
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日期:2024-09-05
Nature and Science, 2008;6(3), ISSN 1545-0740, http://www.sciencepub.org, naturesciencej@gmail.com RNA, DNA and protein isolation using TRIzol reagent Ma Hongbao *, Jenny Young **, Cherng Shen *** * Bioengineering Department, Zhengzhou University ......