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One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in
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日期：2025-09-15
We have developed a simple, one-step procedure for the preparation of competent Escherichia coli that uses a transformation and storage solution [TSS; 1 x TSS is LB broth containing 10% (wt/vol) polyethylene glycol, 5% (vol/vol) dimethyl sulfoxide, and 50...看更多
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