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Rubidium Chloride Competent Cell Protocol | McManus Lab

Rubidium Chloride Competent Cell Protocol | McManus Lab

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日期:2024-08-06
Step 1: Culture bacteria. Streak/plate bacteria of choice on LB agar plate. Inoculate single colony into starter culture of 20 mL SOC media in 125mL Erlenmeyer flask. Incubate overnight in 30 C or 37 C shaker. Inoculate growth culture 1:100 with starter c...看更多