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How to Make Competent Cells - Protocol Online - Your lab's reference book
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日期：2024-08-05
How to Make Competent Cells Step 1: Take an 100 mL aliquot of frozen cells from the -80 o C and innoculate about 500 ml to 1 L sterile LB broth. DO NOT ADD antibiotic, since these cells do not have an plasmid in them. Work as sterile as possible. Step 2: ...看更多
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