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![Unspecific stimulation with PMA + ionomycin vs. PHA for intracellular cytokine staining of T cells](https://www.iarticlesnet.com/pub/img/site/s_21.jpeg)
Unspecific stimulation with PMA + ionomycin vs. PHA for intracellular cytokine staining of T cells
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日期:2024-07-09
Dear Felix, I have an experience with PMA(10ng/ml) plus Ionomycin (1um) and also with PHA(0.3 ug/ml) on human PBMC activation for 48hr.I have measured different types of cytokine including IFN-gamma in cell couture supernatant by Elisa .in this experience...看更多