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![RT-PCR: Two-Step Protocol - MIT OpenCourseWare | Free Online Course Materials](https://www.iarticlesnet.com/pub/img/site/s_10.jpeg)
RT-PCR: Two-Step Protocol - MIT OpenCourseWare | Free Online Course Materials
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日期:2024-08-31
a) Initial denaturation: 94 C for 4 min b) 30 cycles: Denature at 94 C for 30 sec Anneal at 55 C for 20–30 sec** Extend at 72 C for 45 sec *** c) Final extension: 72 C for 5 min **Start with the annealing temperature suggested by your primer design softwa...看更多